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Catalog# Product Description |
ICP0705 This high affinity anti-HA antibody is developed using HA peptide (YPYDVPDYA) conjugated to KLH and affinity-purified with the HA peptide. The antibody is useful for detection, isolation and localization of the HA-tag proteins. |
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Western-blot analysis of the HA-tag protein (50 kd) expressed cell lysate and affinity enriched by IP, with HA-tag antibody, HRP conjugates (ICP0751) 1: Molecular marker 2: 10 uL of HA-tag protein in lysate (original cell lysate) 3: 10 uL of His-tag protein in lysate (after IP depletion) 4: 10 uL of the HA-tag protein in 100 uL of the eluting buffer enriched with 25 ug of the anti-His tag (ICP0750) from 2 mL of the original lysate.
Western blot analysis of the cell extract with expression of HA-tag proteins (B-F) and without expression of HA (A).
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Species
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Rabbit
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Formulation
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500 µg/mL in PBS, 50% glycerol
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Immunogen
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GST peptide EEHLYERDEGDKWR KLH conjugates
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Purification
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Antigen-specific affinity chromatography
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Specificity
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The antibody recognizes HA-tag proteins.
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Applications
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ELISA (1:2500); WB (1:1000); IP (5 µg/sample)
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Scientific Description
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Hemaglutinin peptide (HA) is frequently used as an affinity tag to facilitate the detection, isolation and localization of the HA-fusion proteins.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C.Expiration date is one year from date of shipping if stored properly.
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Product Specific References
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1. Cancer Res. 2010. 70: 10445.doi: 10.1158/0008-5472.CAN-10-3040
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